Figure 1.

Genetic analysis of iron uptake genes of AMA16 (A), AB0057 (B), and AB5075 (C) A. baumannii strains and qRT-PCR of genes associated with iron uptake, pirA, piuA, and bauA, expressed in LB, LB supplemented with HPF or with HSA, or cultured in HS. Fold changes were calculated using double ΔCt analysis. At least three independent samples were used, and four technical replicates were performed from each sample. The LB was used as reference. Statistical significance (p < 0.05) was determined by ANOVA followed by Tukey’s multiple comparison test: one asterisk: p < 0.05; two asterisks: p < 0.01; and three asterisks: p < 0.001.