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. 2022 Mar 18;11(3):583. doi: 10.3390/antiox11030583

Figure 5.

Figure 5

Knockdown of BCAT1 or BCAT2 aggravated ferroptosis and restrained the Keap1/Nrf2/HO-1 signaling pathway in H9c2 cardiomyocytes. (A) Western blotting analysis of BCAT1 and BCAT2 in H9c2 cardiomyocytes transfected with BCAT1 siRNA or BCAT2 siRNA (n = 3–4). (B) The cell viability of H9c2 cardiomyocytes transfected with BCAT1 siRNA or BCAT2 siRNA (n = 6). (C) The content of total iron of H9c2 cardiomyocytes transfected with BCAT1 siRNA (n = 6). (D) Western blotting analysis of GPX4, ACSL4, and FTL in H9c2 cardiomyocytes transfected with BCAT1 siRNA (n = 3). (E) The content of total iron of H9c2 cardiomyocytes transfected with BCAT2 siRNA (n = 6). (F) Western blotting analysis of GPX4, ACSL4, and FTL in H9c2 cardiomyocytes transfected with BCAT2 siRNA (n = 3). Western blotting analysis of Keap1, Nrf2 and HO-1 in H9c2 cardiomyocytes transfected with (G) BCAT1 siRNA or (H) BCAT2 siRNA (n = 3). Results were expressed as mean ± SD. # p  <  0.05, ## p  <  0.01 vs. the control group, & p  <  0.05, && p  <  0.01 vs. the OGD group, @ p  <  0.05, @@ p  <  0.01 vs. the group treated with BCAT1 siRNA or BCAT2 siRNA alone.