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. 2022 Mar 2;12(3):391. doi: 10.3390/biom12030391

Figure 6.

Figure 6

COL11A1 enhanced the cell stemness via the AKT/GSK-3β/Snail signaling pathway: (A) Western blotting analysis of cluster of differentiation (CD)-24 and CD44expression levels in PANC-1 cells treated with pCMV3-COL11A1 or siCOL11A1; (B) mRNA expression levels of CD24 and CD44 were detected in PANC-1 cells treated with different treatments using real-time polymerase chain reaction (PCR); (C) protein expression levels of CD24 and CD44 in PANC-1 cells transfectedwithpCMV3-COL11A1, LY294002, and siSnail; (D) PANC-1 cells were treated under different conditions and the expression levels of CD24and CD44 on the cell membranes were detected using flow cytometry; (E) flow cytometry was used to detect the percentage ofCD24+/CD44+ cells among PANC-1 cells treated with different treatments; (F) Western blotting was used to detect the expression levels of COL11A1 and EMT markers in PANC-1 and CD24+/CD44+-PANC-1 cells; (G) expression levels of EMT markers in CD24+/CD44+-PANC-1 cells treated with different treatments; (H) colony formation assay was performed in PANC-1 cells with different treatments. (n = 3, * p < 0.05).