Figure 4.

A549 were incubated for 4 h with conditioned medium (CM) from untreated (CM_cont) or S1-treated (CM_S1) monocyte-derived macrophages (MDM), either in the absence or in the presence of the indicated inhibitors, added 30 min before the treatment. The expression of the indicated genes was measured by means of RT-qPCR and calculated relative to CM_cont (=1, dotted line) upon normalization for the housekeeping gene RPL15, as described in Methods. Data are means ± SEM of four determinations, each performed in duplicate. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. CM_S1.