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. 2022 Mar 3;10(3):601. doi: 10.3390/biomedicines10030601

Figure 4.

Figure 4

BGJ 398 increases intracellular accumulation of Calcein, an ABCB1 fluorescent substrate, in ABCB1-overexpressing Tx-R HCC 1806 cells. The intracellular accumulation of Calcein, a fluorescent product of the ABCB1 substrate Calcein AM, was determined in the Tx-R HCC 1806 cells (B) and the corresponding drug-sensitive parental HCC 1806 cells (A). The cells indicated above were treated with 100 nM of Calcein AM alone (green) or in combination with 20 µM of BGJ 398 (red) or 50 µM PD 173074 (gray). The fluorescence intensity was analyzed by FACs. Representative hist grams of at least 3 independent experiments are shown.