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Increased levels of reactive oxygen species (ROS) in the MDA-MB-231 line. (A) Confocal microscopy images of mitochondrial ROS were stained by MitoSOX red (red) in MCF10A, MCF7 and MDA-MB-231 cell and total nuclei were stained with Hoechst (blue). (B) ROS production was quantified as MitoSOX red fluorescence intensity/cell ratio. Data were obtained from three biological replicates. The data are presented as mean ± SD of cells (n = 153–200). * p < 0.05, ** p < 0.01.
