Figure 4.

RGC degeneration in egl1 mice. (A) Quantification of RGCs was performed before and at 2 and 8 weeks after IOP elevation. Significant RGC loss was observed after 8 weeks of IOP elevation. Data are presented as means ± SEM (n = 11–17). **: p < 0.01. (B) Representative images show RBPMS (red) immunolabeled retinal whole mounts from mice before (left) and 8 weeks after (right) IOP elevation. The density of RGCs, demonstrated by RBPMS positive cells, decreased after 8 weeks of IOP elevation. Scale bar = 50 µm. (C) Individual RGC count was plotted against IOP exposure described as AUC. There was a moderate negative correlation between RGC counts and IOP exposure. R square: 0.43, p < 0.001.