Figure 2.

HDF cells were seeded in a Seahorse XFp miniplate and treated with 100 μM H₂O₂ for 1 h, followed by medium change and treatment with hASC exosomes (Exo) or exosomes depleted of GAS5 (Exo-G5) for 18 h. A Mito Stress Test Assay was performed according to the manufacturer’s instructions and repeated three times. The readings were normalized to the protein content of each well. Seahorse Wave software was used for analysis of oxygen consumption rate (OCR), basal respiration, percent spare respiratory capacity and coupling efficiency (n = 3). Statistical analysis was performed by one-way ANOVA and significant p-values (<0.05) are indicated on the graph.