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. 2022 Mar 1;10(3):580. doi: 10.3390/biomedicines10030580

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Drug-susceptibility profile of selected VV2 HPMPDAP-R viral clones to evaluate the impact of the D480Y, A684V, and A705T DNA polymerase substitutions. The source of the viral clones was as follows: D480Y (VV2 HPMPDAP-R # 4), and D480Y + A684V + A705T (VV2 HPMPDAP-R #36). Drug-resistance properties of the different types of viral clones were determined using a CPE reduction assay with HEL fibroblasts. The effects of different drugs on viruses encoding the indicated mutations were determined by calculating the EC₅₀ values for the parental wild-type strain and clones bearing the specific mutations. At least two independent experiments were performed for each test compound. Horizontal lines for each drug and mutant viral clones indicate the mean values ± standard deviation. The fold resistance (ratio of the EC₅₀ for the mutant viruses to the EC₅₀ for the corresponding wild-type virus is marked at the top of the graph. VACV viral clones showing a ≥2-fold increase (red, bold) were considered drug resistant (R) and those with a ≤0.5-fold decrease (orange, bold) drug hypersensitive (hs).

Drug-susceptibility profile of selected VV2 HPMPDAP-R viral clones to evaluate the impact of the D480Y, A684V, and A705T DNA polymerase substitutions. The source of the viral clones was as follows: D480Y (VV2 HPMPDAP-R # 4), and D480Y + A684V + A705T (VV2 HPMPDAP-R #36). Drug-resistance properties of the different types of viral clones were determined using a CPE reduction assay with HEL fibroblasts. The effects of different drugs on viruses encoding the indicated mutations were determined by calculating the EC₅₀ values for the parental wild-type strain and clones bearing the specific mutations. At least two independent experiments were performed for each test compound. Horizontal lines for each drug and mutant viral clones indicate the mean values ± standard deviation. The fold resistance (ratio of the EC₅₀ for the mutant viruses to the EC₅₀ for the corresponding wild-type virus is marked at the top of the graph. VACV viral clones showing a ≥2-fold increase (red, bold) were considered drug resistant (R) and those with a ≤0.5-fold decrease (orange, bold) drug hypersensitive (hs).