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. 2022 Mar 1;10(3):580. doi: 10.3390/biomedicines10030580

Table 1.

Identification of amino acid changes in the viral DNA polymerase following selective pressure with HPMPA, cHPMPA, and cHPMPC in VACV Lederle strain.

Drug Amino Acid Changes in Viral DNA Polymerase
Known Natural Occurring Genetic Polymorphisms Known Drug-Resistant Mutations (Dark Blue) and Novel Mutations (Yellow)
HPMPA T845M, G857R, 936–938 ANV A314T
cHPMPA T845M, G857R, 936–938 ANV A353V S851Y
cHPMPC T845M, G857R, 936–938 NΔG A314T

The complete DNA pol (E9L gene) was genotyped for one of the viral clones isolated from each selection procedure with the indicated drug. The presence of the mutations was verified in additional viral clones, encompassing 8 viral clones selected under HPMPA and cHPMPA and 9 viral clones under cHPMPC.