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Effects of S100A9 on proliferation and apoptosis of EECs. (A) Impact of HS on expression of S100A9. (B) Relative expression of S100A9 transfected with pcDAN3.1(+)-S100A9, pcDNA3.1(+), siS100A9, and NC at 24 h post transfection in EECs. (C,D) Flow cytometry analysis of cell cycle distribution. (E,F) Apoptosis by flow cytometry. * p < 0.05, ** p < 0.01 and *** p < 0.001. G1, first gap; S, synthesis; G2, second gap; NC, negative control.
