Skip to main content
. 2022 Mar 8;14(6):1372. doi: 10.3390/cancers14061372

Figure 3.

Figure 3

Effect of celastrol (CEL) (A) and resveratrol (RSV) (B) on the frequency of apoptotic and necrotic cells in LoVo and LoVo/DX cell cultures after 24 h of incubation. The cells were double-stained with Annexin V Alexa Fluo®r 488 and PI fluorescent dyes, and analyzed by flow cytometry. Control: cells incubated with the solvent (DMSO). The results are presented as a percentage of early apoptotic cells (Annexin V Alexa Fluor® 488+ and PI−) and late apoptotic cells (Annexin V Alexa Fluor® 488+ and PI+) and necrotic (Annexin V Alexa Fluor® 488+ and PI+). The results are the mean ± SD of at least three independent experiments. The significance of the differences was determined by Student’s t-test. * p < 0.05, ** p < 0.01, *** p < 0.001.