Figure 4.

Characterization of β’2β’3-cop double mutants. (A) All the β’2β’3-cop double mutants show a wild type phenotype at 7-day-old seedling stage. (B) RT–qPCR analysis show the expression levels of the three β’-COP genes. (C) β’2β’3-cop-1, β’2β’3-cop-2 and β’2β’3-cop-3 mutants show upregulation of the COPII subunit SEC31A gene. Expression of SEC31A and SEC31B was analyzed by RT–qPCR. Total RNA was extracted from 7-day-old seedlings of the mutants and wild type (Col-0). The mRNA was analyzed by RT–qPCR with specific primers and normalized to UBQ10 expression (Supplementary Tables S1–S3). Results are from three biological samples and three technical replicates. mRNA levels are expressed as relative expression levels and represent fold changes of mutant over wild type. Values represent mean ± s.e.m. of the three biological samples.