Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 10;11(6):951. doi: 10.3390/cells11060951

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Light-induced changes of calcium transients and action potential of hiPSC-CMs in vitro. (A) Calcium mapping. Spontaneous calcium transients in hiPSC-CCND2^OE/Luci^OECMs (upper trace) and hiPSC-CCND2^OE/ChR2^OECMs (middle and bottom traces). Blue light illumination (1 s) did not alter rhythmicity of calcium transients in the hiPSC-CCND2^OE/Luci^OECMs, whereas it delayed the subsequent cycle in hiPSC-CCND2^OE/ChR2^OECMs. (B) Voltage mapping. Spontaneous action potentials in hiPSC-CCND2^OE/ChR2^OECMs. Since RH-237 fluorescence decreases with depolarization, the peak and trough fluorescence intensities correspond to the maximal diastolic potential and peak action potential amplitude, respectively. Blue light illumination delayed the onset of the subsequent cycle. Contamination of the fluorescence signals by the blue excitation light prevents their proper interpretation during illumination.