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. 2022 Mar 10;11(6):951. doi: 10.3390/cells11060951

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Immunohistological analysis of engrafted hiPSC-CMs. (A) Heart sections from light-responsive mice that had been sacrificed 6 months after myocardial infarction induction and treatment with PBS and hiPSC-CMs were stained for the presence of hcTnT. Nuclei were counterstained with DAPI. Scale bar = 100µm. (B,C) The engraftment rate was calculated by dividing the number of cells that expressed hcTnT by the total number of cells administered and expressed as a percentage. *—p < 0.05 versus mice receiving hiPSC-CCND2^OE/ChR2^OECMs but not responsive to light; Student’s t-test. n = 10 mice in hiPSC-CCND2^OE/Luci^OECMs group; n = 10 mice in hiPSC-CCND2^OE/ChR2^OECMs group (n = 6 not responsive to light and n = 4 responsive to light). n.s. indicates no significant difference. (D) Engrafted hiPSC-CCND2^OE/ChR2^OECMs were identified in mouse hearts at 6 months after myocardial infarction induction and cell transplantation via immunofluorescent staining for the expression of human cTnT. Both transplanted cardiomyocytes and native cardiomyocytes were stained with antibodies against Connexin-43. Images were from heart sections of light-responsive mice. Scale bar = 100 µm.