Table 1.
Post-translational modification sites in human ubiquitination proteins #.
| Gene | Protein | UniProt | Acetylation + | Phosphorylation + | Ubiquitination + | Disease |
|---|---|---|---|---|---|---|
| Ubiquitin | ||||||
| UBC | Ubiquitin | P0CG48 | K6, K11, K27, K33, K48, K63 | T7, T12, T14, S20, T22, T55, S57, Y59, S65, T66 | M1, K6, K11, K27, K29, K33, K48, K63 | Multiple myeloma, leukemia, colorectal cancer |
| E1 Activating Enzyme | ||||||
| UBA1 | UBE1 | P22314 | K68, K89, K185, K385, K465, K470, K526, K528, K593, K604, K627, K657, K671, K746, K838, K843, K884, K980, K984, K1024 | S3, S4, S7, S13, S21, S24, S28, S31, S46, Y55, S56, Y60, S74, S140, T191, Y273, T274, S284, Y286, S293, S305, S309, T318, Y388, S460, Y560, Y590, T600, T603, T615, S628, T682, S781, T789, S793, T800, S803, S810, S816, S820, S824, S835, S855, S866, Y873, Y978, S1044 | K68, K89, K97, K185, K296, K299, K304, K322, K385, K411, K416, K443, K465, K468, K470, K526, K528, K593, K604, K627, K635, K657, K671, K746, K802, K806, K830, K838, K843, K889, K923, K980 | Melanoma, lymphoma, leukemia, breast, colorectal, and gastric cancers |
| E2 Conjugating Enzymes | ||||||
| UBE2A | UBE2A, RAD6A | P49459 | K66 | S120, S142, S148 | K66, K75 | Breast, lung cancer |
| UBE2B | UBE2B, RAD6B | P63146 | K66 | T69, S120 | K66 | Leukemia |
| UBE2C | UBE2C, UbcH10 | O00762 | K18, K119, K121, K164 | S3, T11, S51, S87, T160 | K80, K119, K121, K164, K172 | Leukemia |
| UBE2D1 | UbcH5a | P51668 | K144 | S83, Y145 | K144 | Lung cancer |
| UBE2D2 | UbcH5b | P62837 | K8, K144 | S83, S94, T98 | K8, K101, K128, K144 | Lung cancer |
| UBE2D3 | UbcH5c | P61077 | K8, K133, K144 | S11, S83, S94, T98 | K8, K101, K128, K133, K144 | Breast, lung cancers, leukemia |
| UBE2D4 | UbcH5d | Q9Y2X8 | K8, K144 | K144 | Leukemia, multiple myeloma | |
| UBE2E1 | UbcH6 | P51965 | K43, K50, K136 | S2, S6, S9, T28, S46, Y77 | K24, K43, K50, K54, K72, K136 | Breast, colorectal cancers, leukemia |
| UBE2E2 | UbcH8 (ISG15) | Q96LR5 | K48, K52 | T3, S11, S13, T14, S15, S18, S19, T49, S54, Y85 | K62, K144 | Breast, colorectal, lung, gastric cancers, leukemia |
| UBE2E3 | UbcH9 | Q969T4 | K50, K58 | S3, S8, S12, S19, Y91 | K39, K58, K68, K150 | Breast, lung, gastric cancers, leukemia |
| UBE2F | UBE2F, NCE2 (NEDD8) | Q969M7 | K7, K9 | S31, T85, S124, Y179 | K7 | Breast, brain, lung, gastric cancers |
| UBE2G1 | Ubc7 | P62253 | T2, S6, Y65, T76, Y102, Y104 | K19, K63, K73, K101, K106, K163 | Multiple myeloma | |
| UBE2G2 | Ubc7 | P60604 | K7, K142, K153, K156, K161 | Leukemia | ||
| UBE2H | UbcH2, E2-20K | P62256 | K8, K60, K64, K147 | S2, S3, S5, T13, S65, S166, S169 | K17, K60, K64, K147 | Breast cancer |
| UBE2I | Ubc9 (SUMO) | P63279 | K30, K48, K59, K65, K74, K146 | T35, S70, S71 | K18, K49, K59, K65, K74 | Bone cancer, leukemia |
| UBE2J1 | NCUBE1 | Q9Y385 | K8, K17 | Y5, S9, S51, S184, S251, S266, T267, S268, T282, T295, Y307, Y312 | K8, K143, K164, K177, K186, K194 | Breast, lung cancers, leukemia, multiple myeloma |
| UBE2J2 | NCUBE2 | Q8N2K1 | K18 | Y31, Y46 | K18, K64, K139, K152, K154, K168 | Esophageal cancer |
| UBE2K | E2-25K, HIP-2 | P61086 | K14, K18, K72, K142, K164, K165 | T49, S158 *, S159, Y162 *, T163 *, S185 | K14, K18, K24, K28, K61, K72, K97, K142, K164, K165 | Leukemia, lung cancer |
| UBE2L3 | UbcH7 | P68036 | K9, K20, K64, K73, K82, K96, K131, K138, K145 | Y75, Y129, Y147 | K9, K20, K48, K64, K67, K71, K73, K82, K96, K100, K131, K138, K145, K150 | Lung, colorectal cancers, lymphoma, leukemia |
| UBE2L6 | UbcH8 (ISG15) | O14933 | K138 | S26, S153 | K9, K16, K17, K96, K138 | Breast cancer, leukemia, neuroblastoma |
| UBE2M | Ubc12 (NEDD8) | P61081 | K3, K8, K36, K45, K72 | S6, T20, S23, S28, T46, S50, S52 *, Y86, Y172, Y177 | K3, K8, K26, K36, K45, K61, K72, K75, K81, K92, K94 | Breast, lung cancers, leukemia, lymphoma |
| UBE2N | Ubc13 | P61088 | K10, K24, K53, K82, K92, K94 | Y34, S45, T139, T144, Y147 | K10, K24, K68, K74, K82, K92, K94 | Breast, lung, gastric, colorectal cancers, leukemia, lymphoma |
| UBE2O | E2-230K, KIAA1734 | Q9C0C9 | None in UBC | None in UBC | K953, K990, K1038 | Leukemia, lymphoma |
| UBE2Q1 | UBE2Q, NICE5 | Q7Z7E8 | K403 | Y264, S391, Y393, S394, S401, S404, Y415 | K307, K390, K403 | Breast cancer |
| UBE2Q2 | UBE2Q2 | Q8WVN8 | S357, Y368, T369 | Breast, gastric cancers | ||
| UBE2R1 | Cdc34, UBCH3B | P49427 | K167, K173 | Y68, S71, T89, T162, Y190, S203, S222, S231, T233, S236 | K11, K18, K63, K157, K167, K182, K193 | Bone, colorectal, cervical cancers |
| UBE2R2 | Cdc34B, UBC3B | Q712K3 | Y190, S202, Y207, Y228, S233, S238 | K11, K18, K63, K157, K159, K167, K173, K182, K193, K195 | Leukemia | |
| UBE2S | UBE2S, E2-24K | Q16763 | K68, K82 | S73, Y78, T81, T152, S173, S175, T180 | K18, K32, K63, K68, K76, K100, K117, K197, K198 | Prostate, gastric cancers, leukemia |
| UBE2T | UBE2T | Q9NPD8 | K28, K91, K191 | T72, S172, S177, T178, S184 | K28, K48, K91, K136, K141, K156, K182, K191, K192 | Breast, lung cancers, leukemia, multiple myeloma |
| UBE2U | UBE2U | Q5VVX9 | None in UBC | |||
| UBE2V1 | UEV1 | Q13404 | K10, K24, K30, K74 | S7, T86, S106, Y145, S146 | K10, K68, K74, K131 | Breast, lung cancers, leukemia |
| UBE2V2 | UEV2, MMS2 | Q15819 | K8, K66, K72, K108 | S4, T5, S79, S102 | K8, K66, K72, K108, K129, K133 | Breast, colorectal cancers, leukemia |
| UBE2W | UBC16 | Q96B02 | S29, S33 | K10 | ||
| UBE2Z | UBE2Z, USE1(FAT10) | Q9H832 | K166, K238 | None in UBC | K113, K166, K238 | Leukemia |
# Post-translational modification sites have been curated from references [8,9,10,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,72,73,74,75,76,77,78,79,80,81,82,83,84,85,86,87,88,89,90]. For specific sites readers are encouraged to review data in the original references. + Only post-translational modifications in the UBC fold region are listed. In many cases there are extensive modifications in accessory regions/domains. * Some sites have low probabilities.