Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 15;12(3):455. doi: 10.3390/biom12030455

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Scheme A2 — Effect of DMSO on calpain activity, PARP activity, and TUNEL staining in rd1 retinal explants. Calpain activity (blue), PARP activity (green), and TUNEL (magenta) were used in rd1 retinal explant cultures. ToPro (red) and DAPI (grey) were used as nuclear counterstains. rd1 control retina (untr.; a,d,g) was compared to retina treated with 0.1% DMSO (DMSO; b,e,h). There was no difference between positive cells detected in the rd1 outer nuclear layer (ONL), with or without DMSO. The scatter plot (e) shows the percentage of calpain activity (c), PARP activity (f), and TUNEL-positive cells (i). In the calpain activity assay, untr. rd1: 16 explants derived from 16 different animals; DMSO rd1: 6/6. In the PARP activity assay, untr. rd1: 18/18; DMSO rd1: 6/6. In the TUNEL assay, untr. rd1: 27/27; DMSO rd1: 6/6; error bars represent SD; ns = p > 0.05, INL = inner nuclear layer, GCL = ganglion cell layer. Scale bar = 50 µm.