Figure 5.

Efgartigimod treatment protects keratinocyte monolayers against dissociation induced by PV IgG. hTert keratinocytes were plated on 24-well plates, grown in KGM2 medium with 0.05 mM CaCl₂ until confluent, and then switched to KGM2 with 2 mM CaCl₂ for 24 h. The cells were left untreated, or efgartigimod (25 µg/mL) was applied for 30 min prior to the 24 h treatment with hAK23 (12.5 µg/mL), control IgG4 (12.5 µg/mL), PV IgG (150 µg/mL) or human control IgG (150 µg/mL). Monolayer dissociation assay was performed in triplicates, and the number of fragments was quantified using ImageJ software. A representative experiment is shown. The error bars show the SD of the mean values obtained from five independent experiments. Statistical analysis was done using two-way ANOVA with Bonferroni’s post-test. Statistically significant differences are indicated by *** = p ≤ 0.001.