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. 2022 Mar 15;14(6):1499. doi: 10.3390/cancers14061499

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Differentiation Analysis of NG108-15 Cells Reveals that Treatments with Pantoprazole Increased Neuronal Markers after 6 days. Immunofluorescence of cells was analyzed with CellProfiler and quantified for integrated fluorescence intensity. (A) Stain of Microtubule Associated Protein 2 (MAP2). (B) Stain of Neuron-Specific Class III β-Tubulin (Tuj I). (C) Stain of Neural Filament Medium Chain (NFM). (D) Stain of Neuron-Specific Enolase (NSE). Treatments corresponding to the colored bars are outlined in the figure itself. The log of the fold change in intensity was compared between single treatments and combined treatments, except the positive control, with significant values shown in the color of the treatment compared. The initial fluorescence intensities were compared to their corresponding control, with significant values shown under the bars as, ***: p < 0.001, **: p < 0.01, *: p < 0.05 (one-way ANOVA with Tukey post hoc analysis n > 3 technical replicates).