Figure 12.

Differentiation Analysis of U87 Cells Reveals that Treatments with Pantoprazole Increased Astrocytic and Differentiation Markers after 6 days. Immunofluorescence of cells was conducted and analyzed with CellProfiler for integrated fluorescence intensity. (A) Stain of Vimentin. (B) Stain of the phosphorylated cAMP-Response Element Binding Protein (CREB). (C) Stain of S100 calcium binding protein B (S100B). (D) Stain Glial Fibrillary Acidic Protein (GFAP). Treatments corresponding to the colored bars are outlined in the figure itself. The log of the fold change in intensity was compared between single treatments and combined treatments, except the positive control, with significant values shown in the color of the treatment compared. The initial fluorescence intensities were compared to their corresponding control, with significant values shown under the bars as, ***: p < 0.001, **: p < 0.01, *: p < 0.05 (one-way ANOVA with Tukey post hoc analysis n > 3 technical replicates).