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. 2022 Mar 2;12(3):154. doi: 10.3390/bios12030154

Figure 3.

Figure 3

sgRNA screening. (A) Endpoint CRISPR/Cas12a for sgRNA screening. Six sgRNAs were designed based on the target DNA and used in CRISPR/Cas12a at 37 °C for 30 min, and sgRNA 4 worked well in the standard program, with strong fluorescent light under LED blue light. (B) Real-time CRISPR/Cas12a for sgRNA screening. The real-time monitoring was conducted in a CFX96 Touch Real-Time PCR Detection System for 30 min, and the endpoint fluorescence intensity and time with a fluorescence threshold of 1000 were monitored. Each experiment was repeated three times with similar results.