Skip to main content
. 2022 Mar 2;12(3):154. doi: 10.3390/bios12030154

Figure 5.

Figure 5

Specificity evaluation ICB-LAMP-CRISPR/Cas12a method. The specificity of ICB-LAMP-CRISPR/Cas12a was compared with PCR, qPCR, and LAMP. C. jejuni NCTC 11168 and 6 non-C. jejuni (C. coli, E. coli, S. flexneri, K. pneumoniae, P. mirabilis, and S. enteritidis) were used as the tested sample. The specificity evaluation results of PCR and LAMP were shown by agarose gel electrophoresis. The specificity evaluation results of qPCR and ICB-LAMP-CRISPR/Cas12a are shown by the real-time amplification curve and end-point fluorescence. (A) The specificity evaluation of PCR. (B) The specificity evaluation of qPCR. (C) The specificity evaluation of LAMP. (D) The specificity evaluation of the ICB-LAMP-CRISPR/Cas12a method.