Figure 1.

Fibers and Schwann cell nuclei in Wt and TrJ sciatic nerves. (A): Confocal images of Wt and TrJ sciatic nerve fibers. (B–D): Box plots illustrating the distribution of number of fibers, and SC nuclei through the whole thickness of sections and SC nuclear volumes (µm³) of Wt and TrJ, respectively. The fibers were immunostained using anti-PMP22 (green), after epitope retrieval (70% formic acid), and counterstained with DAPI (blue). Both areas and volumes were measured on DAPI channel. Boxes enclose the data comprising between the 25th and 75th percentiles (50% of the data). The median (50th percentile) is denoted by the transversal line within each box. Differences between Wt and TrJ were examined by means of Mann–Whitney tests (**** p-Values < 0.0001). Number of panoramic images analyzed = 20 per genotype. Volumes were calculated in 150 SC nuclei per genotype. Scale bar = 2 µm. Both the fiber and SC nucleus numbers were significantly larger in TrJ than in Wt sciatic nerves, whereas SC nuclear volume was significantly smaller in TrJ than Wt mice.