Figure 1.

MC degranulation via MRGPRX2 relies on G_αi, G_αq and calcium mobilization. (A–C) Cultured or (D–F) ex vivo skin-derived MCs were pretreated with 200 ng/mL PTX, 10 µM YM-254890, 100 µM 2-APB or 1 µM La3+. Then cells were triggered by 10 µg/mL c48/80, 30 µM SP or IgER-CL (cross-linking) (0.1 µg/mL anti-FcεRI-antibody AER-37), β-hexosaminidase release was determined as described in methods. Data are from 5–11 independent experiments, ns: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, skMCs: skin mast cells.