Figure 5.

Ca++ channel inhibition boosts AKT activation downstream of IgER-CL. MCs were pretreated with inhibitors, as described in Figure 3, then stimulated for 30 min with AER-37 (0.1 µg/mL) for FcεRI-aggregation. (A–C) Signals of pERK1/2, pp38 and pAKT were quantified and normalized to cyclophilin B as described in methods. (D–F) Representative blots of A–C. n = 10–13, Mean ± SEM. * p < 0.05, *** p < 0.001, **** p < 0.0001.