Skip to main content
. 2022 Mar 12;14(6):1460. doi: 10.3390/cancers14061460

Figure 4.

Figure 4

Non-toxic combination of ABT-263 and Olaparib can synergize after X-ray and proton irradiation to increase clonogenic cell death. A549, HCT-116 and KP4 cells were irradiated with 3 and 5 Gy X-rays or 1 and 2.5 Gy protons with or without 0.5 µM of Olaparib. The medium was replaced 22 h after irradiation, and 1 µM of ABT-263 was added. Colonies were stained and counted 8 days (KP4 cells) or 12 days (A549, HCT-116 cells) post-irradiation. (a) A549 cells after X-rays. (b) A549 cells after protons. (c) HCT-116 cells after X-rays. (d) KP4 cells after X-rays. At least three independent experiments were performed, and data are presented as mean ± 1 SD. Unpaired t-tests were performed (*: p < 0.05; **: p < 0.01; ***: p < 0.001) for CTL vs. ABT, CTL vs. Ola and Ola vs. Ola + ABT.