Figure 5.

ABT-263 reduces the SASP promoted by Olaparib and radiations in A549 cells. A549 cells were irradiated with 5 Gy X-rays or 2.5 Gy protons with or without 0.5 µM of Olaparib. The medium was replaced 22 h after irradiation, and 1 µM of ABT-263 was added. (a,b) 3 days post-irradiation, mRNA level of genes implicated in SASP were determined. Fold changes, calculated as 2^−ΔΔct, are presented after X-rays (a) and protons (b) radiation. (c) Relative secretion of IL6 six days after X-rays. At least three independent experiments were performed, and data are presented as mean ± 1 SD. Unpaired t-tests were performed (*: p < 0.05; **: p < 0.01) for CTL vs. ABT, CTL vs. Ola and Ola vs. Ola + ABT.