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. 2022 Feb 28;12(3):325. doi: 10.3390/brainsci12030325

Figure 1.

Figure 1

Immunohistochemical detection and quantification of Syn II in the CA1 region of the hippocampus. (A) Horizontal slices through the middle hippocampus stained using a fluorescent Nissl (Neurotrace 500/525) to provide an anatomical reference point and immunolabeled for Syn II. Green channel (left): Nissl staining shows similar anatomy for the WT and Syn2 KO slices. Red channel (right): immunolocalization of Syn II. The left and right panels show identical fields of view. Note a robust Syn II signal detected in the WT slice but only background fluorescence in the Syn2 KO slice. Scale bars: 200 µm. (B) The fluorescence intensity line profiles for the Syn II signal (red channel) over the different cell layers of the CA1 region for these slices as denoted by the yellow lines in panel A. (C) The distributions of pixel flurescence intensities for the anti-Syn II signal (red channel) for WT and Syn2 KO slices. Note a single peak (background) for the Syn2 KO strain and two peaks (background and Syn II signal) for WT. p < 0.001 per K.-S. test. (D) Syn II immunolabeling has a punctate pattern in WT slices, as expected from Syn II being locatized to nerve terminals. Scale bar: 10 µm.