Table 1.
Applications of N-glycan IMS
| Disease | Year | Group | Cohort | Disease-Associated N-glycans |
|---|---|---|---|---|
| Cancer | ||||
| Breast | 2019 | Scott et al. | Whole tissue, TMA | Polylactosamines ↑ (HER2 and TN-associated) |
| 2019 | Herrera et al. | Whole tissue, TMA | Tetra-antennary with LacNAc ↑ (poor survival-associated) | |
| 2019 | Scott et al. | Whole tissue | Sialylation ↑ Fucosylation ↓ (necrosis-associated) | |
| 2019 | Angel et al. | 4T1 cells | Hex9HexNAc2 ↑ | |
| Colorectal | 2016 | Holst et al. | Whole tissue | Sialylation ↑ |
| 2016 | Heijs et al. | Whole tissue | High mannose ↑ | |
| 2020 | Boyaval et al. | Whole tissue | High mannose ↑ | |
| Sialylation ↑ | ||||
| Lung | 2017 | Drake et al. | TMA | High mannose ↑ |
| Hex6HexNAc2 ↑ | ||||
| 2020 | Carter et al. | Whole tissue | High mannose ↑ (hyperplasia-associated) | |
| Fucosylated biantennary ↑ (hyperplasia-associated) | ||||
| Liver | 2014 | Powers et al. | TMA | Hex7HexNAc6 ↑ |
| Hex8HexNAc2 ↑ | ||||
| 2015 | Powers et al. | Whole tissue, TMA | Hex7HexNAc6 ↑ | |
| Core fucosylation ↑ | ||||
| 2018 | West et al. | Whole tissue, TMA | Hex7HexNAc6 ↑ | |
| Branching ↑ | ||||
| Fucosylation ↑ (poor survival-associated) | ||||
| 2019 | Angel et al. | HepG2/C3A cells | Afucosylated bi-/tri-antennary ↑ | |
| Hex9HexNAc2 ↑ | ||||
| 2020 | West et al. | Whole tissue, TMA | Core fucosylation ↑ (poor survival-associated) | |
| 2020 | Black et al. | Serum | Hex3dHex1HexNAc4 ↑ (cirrhosis-associated) | |
| Hex5HexNAc4NeuAc2 ↑ (cirrhosis-associated) | ||||
| Ovarian | 2016 | Everest-Dass et al. | Whole tissue | High mannose ↑ |
| 2019 | Briggs et al. | Whole tissue, TMA | Hex9HexNAc2 ↑ (stage III-associated) | |
| Fucosylation ↑ (stage III-associated) | ||||
| Bisecting GlcNAc ↑ (stage III associated) | ||||
| Sialylation ↑ (stage III-associated) | ||||
| Pancreatic | 2014 | Powers et al. | Whole tissue | Biantennary ± fucose ↑ (fibrosis-associated) |
| Undecorated ↑ (necrosis-associated) | ||||
| 2015 | Powers et al. | Whole tissue | Biantennary ↑ (fibrosis-associated) | |
| 2020 | McDowell et al. | Whole tissue, TMA | Fucosylation ↑ | |
| Branching ↑ | ||||
| Bisecting GlcNAc ↑ | ||||
| Sialylation ↑ | ||||
| Polylactosamines ↑ (tumor margin-associated) | ||||
| Undecorated ↑ (necrosis-associated) | ||||
| High mannose ↓ | ||||
| Sulfated, terminal GalNAc biantennary ↑ (islet-associated) | ||||
| Renal | 2019 | Drake et al. | Whole tissue, TMA | Bisecting GlcNAc ↑ (proximal tubule-associated) |
| Hex7dHex1HexNAc6 ± sialic acid ↑ (glomeruli-associated) | ||||
| Polylactosamine ↑ | ||||
| Branching ↑ | ||||
| Outer-arm fucosylation ↓ | ||||
| Hex5dHex1HexNAc4NeuAc1 ↑ (fibrillar capsule-associated) | ||||
| Fucosylation ↓ (PKD cyst fluid-associated) | ||||
| Non-Cancer | ||||
| Osteoarthritis | 2016 | Briggs et al. | Whole tissue | Hex5HexNAc4NeuAc2 ↑ (bone marrow lesion-associated) |
↑ increased expression relative to normal healthy tissue
↓ decreased expression relative to normal healthy tissue
( ) association with a specific disease or tissue subtype