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. 2022 Mar 10;11(6):955. doi: 10.3390/cells11060955

Figure 1.

Figure 1

Palmitic acid did not impact H3Kac in compressed HPdLFs stimulated with P. gingivalis LPS. (a,b) Quantitative expression analysis of genes encoding histone acetyl transferases (CREBBP, EP300, KAT8, NCOA3) in (a) and genes encoding proteins relevant for histone deacetylation (HDAC1, HDAC2, HDAC3, SIN3A) in (b) in human periodontal ligament fibroblasts (HPdLFs) exposed to palmitic acid (PA) and simultaneously stimulated with compressive force (CF) and P. gingivalis LPS and compared to BSA+LPS controls. (c,d) Representative micrographs of global H3K9/14/18/23/27 acetylation (H3Kac) of HPdLFs under previous conditions. H3Kac staining intensity is shown in thermal LUT (th. LUT) analyzed in relation to BSA+LPS control (c). Cell nuclei were visualized with DAPI. *** p < 0.001 in relation to BSA+LPS; ### p < 0.001 in relation to PA+LPS; n.s., no significant difference between the conditions. One-way ANOVA and post hoc test (Tukey) were used for analysis. Scale bars: 10 μm in (c).