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. 2022 Mar 10;11(6):955. doi: 10.3390/cells11060955

Figure 2.

Figure 2

K27 trimethylation in response to dual stimulation was minor in PA-exposed HPdLFs. (a) Quantitative expression analysis of genes encoding components of the polycomb repressive complex (EZH1, EZH2, SUZ12 and EED) in human periodontal ligament fibroblasts (HPdLFs) exposed to palmitic acid (PA) and simultaneously stimulated with compressive force (CF) and P. gingivalis LPS and compared to the BSA+LPS controls. (b,c) Representative microphotographs of global H3K27me3 of HPdLFs under previous conditions. H3K27me3 staining intensity is shown as thermal LUT (th. LUT) in (c) in relation to BSA+LPS control. DAPI indicates the nuclei. *** p < 0.001 in relation to BSA+LPS; ### p < 0.001 in relation to PA+LPS; §§§ p < 0.001 in relation to BSA+LPS+CF; n.s., no significant difference between the conditions. One-way ANOVA and post hoc test (Tukey) were used for analysis. Scale bars: 10 μm in (b).