Figure 2.

NRF2 is involved in SFN-induced inhibition of CDDP cytotoxicity. (a) NRF2-proficient (NRF2-ctr) and NRF2-KO (NRF2-Cas9) cells were treated with SFN (2 µM) for 8 h and NRF2 and HO-1 protein levels analyzed by Western blot. Actin was used as protein loading control. Densitometric analysis of NRF2/β-actin and HO-1/β-actin is reported in the right panels. * p ≤ 0.01. (b) NRF2-ctr and NRF2-Cas9 cell proliferation (left panel) was measured by XTT assay and cell viability (right panel) was measured by Trypan blue staining after treatment with cisplatin (CDDP) (5 µg/mL) alone or in combination with SFN (2 µM) for 24 h. The results are expressed as cell death percentage ± S.D. * p ≤ 0.01.