FIG. 2.

Cell-free interactions between HOCl and cefoperazone. (A) Effects of different doses of cefoperazone on the recovery of taurine monochloramine (Tau-NHCl) from a mixture of HOCl and taurine. The experiments were carried out by adding ∼35 nmol of HOCl to mixtures of taurine plus cefoperazone (final volume of 1 ml). The taurine concentration was 100 μM (constant). (B) Absorbance spectrum of HOCl alone (- - - ) and in presence of cefoperazone (—). HOCl and cefoperazone concentrations were 1 mM in phosphate-buffered saline, pH 7.4; final solution, pH 7.4.