Figure 2.

Mimetics are not toxic to U251 cells up to 100 µM. U251 cells were seeded at 5 × 10⁴ cells/mL, 100 μL/well in duplicates in 96-well plates. (a) Graph shows percentage of viable cells treated for 24 h with different concentrations of anagrelide, 2-hydroxy-5-fluoropyramidine or mestranol (1, 10, 100 μM) and then stained with calcein-AM and propidium iodide. Five images were taken per well, and values for each condition were averaged. Error bars show mean + SEM. (b) Representative images of untreated cells, vehicle-treated cells and cells treated with 100 μM mimetics. Green cells stained with calcein-AM indicate live cells and red nuclei stained with propidium iodide indicate dead cells. Scale bar for all images, 50 μm in panel for untreated cells. There was no difference between untreated cells and vehicle-treated cells.