Figure 4.

2-Hydroxy-5-fluoropyrimidine inhibits migration of U251 cells. U251 cells were seeded in 96-well plates. After 24 h, monolayers were scratched, immediately imaged, and then treated with different concentrations of 2-hydroxy-5-fluoropyrimidine (1, 10 and 100 μM). Cells were imaged every 24 h up to 96 h. (a) Graph represents the gap width (μm) of untreated cells and cells treated with vehicle or 2-hydroxy-5-fluoropyramidine. Two images were taken per well and values for each condition were averaged. Error bars show mean + SEM from 5 independent experiments. * p < 0.05, # p < 0.01, ^ p < 0.001 as determined by one-way ANOVA with Tukey’s post hoc test. (b) Representative images of 100 μM 2-hydroxy-5-fluoropyrimidine-treated and vehicle-treated cells. Scale bar for all images, 200 μm in panel vehicle, 0 h.