Figure 6.

Mimetics do not inhibit migration of OVCAR3 cells after L1 knockdown. OVCAR3-scrambled and OVCAR3-SH1 cells were seeded in 96-well plates. After 24 h, monolayers were scratched, immediately imaged, and then treated with different concentrations of (a) anagrelide (c) 2-hydroxy 5-fluoropyramidine or (e) mestranol (1, 10 and 100 μM). Cells were imaged every 24 h up to 96 h. Migration of cells treated with mimetics is not inhibited compared to scrambled shRNA-treated cells from 48 h to 96 h. Data show mean + SEM. * p< 0.05, ** p < 0.01 and *** p < 0.001 difference to the respective scrambled shRNA (Scr) control, as determined by one-way ANOVA with Tukey’s post hoc test. (b,d,f) Representative images of OVCAR3 scrambled and SH1 cells treated with 100 μM mimetics. Scale bar for all images, 200 μm in panel (a) scrambled.