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. 2022 Mar 18;11(6):1042. doi: 10.3390/cells11061042

Figure 3.

Figure 3

Doxorubicin enhances the expression of proinflammatory cytokines IL-1β and IL-6 in osteosarcoma. (A) Gene expression of the proinflammatory cytokines IL-1β, IL-6, IFN-γ, TNF-α, and TGF-β after doxorubicin treatment. Human osteosarcoma cells were treated with doxorubicin (40 nM) for 4 days, and qRT-PCR was performed to determine cytokine gene expression (N.D., not detected). The data represent the means ± SD. *, p < 0.05 and **, p < 0.01 vs. control (0.04% DMSO). (B) IL-1β and IL-6 gene expression in MG63 and 143B cells after doxorubicin withdrawal for 10 days. The data represent the means ± SD. *, p < 0.05 and **, p < 0.01 vs. untreated parental cells. (C) Secretion of IL-1β and IL-6 after doxorubicin treatment. Osteosarcoma cells were treated with doxorubicin (40 nM), and the culture media were collected on days 1, 2, 3, and 4 to determine the IL-1β and IL-6 levels using ELISA. The data represent the means ± SD. *, p < 0.05 and **, p < 0.01 vs. control (0.04% DMSO).