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Doxorubicin promotes the release of EVs in osteosarcoma cells. Effect of doxorubicin on EV secretion from osteosarcoma cells. (A) Quantification of EV at days 1, 2, and 4 after doxorubicin treatment. (B) Representative size distribution estimation of EV before and after doxorubicin treatment. (C) Representative dot plot of annexin V+ EV by flow cytometry. The data represent the means ± SD. *, p < 0.05 and **, p < 0.01 vs. control (0.04% DMSO).
