Fig 3. Example Sh-RPA amplification curves using representative PuCVL samples.
Three strong and early-onset positive Sh-RPA amplification curves with relatively low associated real-time PCR Ct values are shown (samples CVL208, CVL210 and CVL500). One weak but positive Sh-RPA amplification curve with a relatively high associated real-time PCR Ct value is shown (CVL125). One weak but positive Sh-RPA amplification curve with an associated real-time PCR Ct value of >50 (and so deemed negative by real-time PCR [40]) is shown (CVL185). Samples CVL164 and CVL212 were deemed negative by both real-time PCR (Ct >50) and Sh-RPA. Neither sample generated a Sh-RPA amplification curve. All real-time PCR Ct values were generated during [40].