Figure 3.

β-arrestins are involved in mGPR1 subcellular localization and trafficking. (A–D) Real-time measurement of BRET signal in WT (●) or β-arrestin 1/2 KO (○) HEK293 cells expressing hGPR1- RLuc or mGPR1-RLuc in combination with KRas-Venus (plasma membrane) (A,B) or Rab5-Venus (early endosomes) (C,D), in cells expressing or not basal conditions and after stimulation with 100 nM chemerin. Results are expressed as Net BRET corresponding to the BRET signal measured between the donor and the acceptor minus the BRET signal measured with the donor only. Data represent the mean ± SEM of at least three independent experiments.