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. 2022 Mar 24;18(3):e1010378. doi: 10.1371/journal.ppat.1010378

Fig 6. Higher levels of the plasma ATP may upregulate the expression of miRNA30b-30e in HIV-infected individuals.

Fig 6

Fold regulation of the CD73 gene in isolated CD8+ T cells after treatment with (A) the miR-30b, (B) miR-30c, (C) miR-30d, and (D) miR-30e inhibitors. (E) Quantification of ATP levels in the plasma of HIV infected individuals versus HCs using ATP light kit. (F) Representative histogram plots of CD73 expression in CD8+ T cells with/without treatment with ATP (25 μM) for 72 hr. (G) Cumulative data showing MFI of CD73 in CD8+ T cells after their treatment with ATP. (H) Cumulative data showing MFI for CD73 in CD8+ T cells treated with or without adenosine (10 μM) for 72 hr. (I) Fold change of TNF-α, and (J) IFN-γ genes in CD8+ T cells treated with or without ATP (25 μM) for 72 hr. (K) Representative flow cytometry plots, and (L) cumulative data for TNF-α, and (M) IFN-γ expression in CD8+ T cells stimulated (stim) with anti-CD3/CD28 antibodies overnight without or with ATP (25 μM). Unstimulated (Unstim) as negative control. (N) Fold change of miR-30b, (O) miR-30c, (P) miR-30d, and (Q) miR-30e in isolated CD8+ T cells after treatment with ATP (25 μM) for 2 hr. Each dot represents data from an individual human subject. Statistical analysis determined by the Mann-Whitney U-test (A-E, H-J, and N-Q), Wilcoxon matched-pairs test (G, L and M) with significance indicated. ns: no significant. n: reflects the number of samples.