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. 2022 Mar 14;18(3):e1009949. doi: 10.1371/journal.pcbi.1009949

Fig 5. Estimation of E2Fs accumulation over the cell cycle without marker identification with respect to nuclei segmentation.

Fig 5

Temporal evolution of E2F3A, E2F8 and E2F4 protein accumulation over the cell cycle in mouse intestinal epithelium, by considering positive/negative states for EdU and pH3 a, by only considering intensity for E2Fs, EdU and pH3 with a manual segmentation for nuclei b, with a nuclei segmentation obtained for the confocal images with a Mask R-CNN model trained with the widefield images and a manual segmentation for the widefield images c, and with a nuclei segmentation obtained for the widefield images with a Mask R-CNN model trained with the confocal images and a manual segmentation for the confocal images d.