Figure 5. Skeletal muscles of the cholinergic neuron-specific Tigar knockout (chTKO) mice at 4°C display increased pentose phosphate pathway, purine nucleotide cycle, and amino acid utilization pathways.
The control ChatCre and chTKO male mice (n = 4–6) were maintained at room temperature (RT) or shifted to 4°C for 1 hr. Quadricep white skeletal muscles were isolated and extracts were subjected to widely targeted (multiple reaction monitoring [MRM]) small metabolite profiling using an ABSciex 6500 + QTRAP with ACE PFP and Merck ZIC-pHILIC columns as described in ‘Materials and methods.’ The heatmap shows the metabolites/pathways differentially identified with the corresponding p-values.
Figure 5—source data 1. Raw metabolites data were collected in the gastrocnemius muscle of both ambient temperature housed and 4°C 1 hr exposed ChatCre and cholinergic neuron-specific Tigar knockout (chTKO) mice as described in ‘Method details’.
The data was used in Figure 5.
elife-73360-fig5-data1.xlsx (22.1KB, xlsx)