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. 2022 Mar 7;11:e73360. doi: 10.7554/eLife.73360

Figure 7. The cholinergic neuron-specific Tigar knockout (chTKO) mice display enhanced cold-stimulated skeletal muscle shivering activity and increased cholinergic tone.

Figure 7.

(A) Male mice core body temperatures (ChatCre n = 7, TKO n = 10), (B) blood pressure (mmHg) (ChatCre n = 7, chTKO n = 10), (C) heart rate (beats per minute, BPM) (ChatCre n = 7, chTKO n = 6), (D) representative electromyography (EMG) traces and root mean squares (RMS) for ChatCre and chTKO male mice when shifted to 4°C for 30 min, and (E) neck EMG (ChatCre n = 5, chTKO n = 6) were measured continuously starting at ambient laboratory temperature (0 min) and for 60 min following placement at 4°C. (F) Extensor digitorium longus (EDL) muscles from two independent control ChatCre and chTKO male mice at room temperature (RT) or shifted to 4°C for 1 hr were isolated, and tissue extracts were immunoblotted with an antibody for the phospho-threonine 172 AMPKα subunit (pT172-AMPKα) or total α subunit (AMPKα). Statistical analyses are described in ‘Method details,’ and the data are presented as the mean ± SD. **p<0.01.

Figure 7—source data 1. The extensor digitorum longus (EDL) muscles were collected from both ambient temperature housed and 4°C 1 hr exposed ChatCre and cholinergic neuron-specific Tigar knockout (chTKO) mice, and 30 μg of the tissue lysate were used for AMPKα (62 kDa) immunoblotting analysis as described in the ‘Immunoblotting’ section.
The raw image was used for Figure 7F. A detailed description of the raw images is shown in Source data 1.
Figure 7—source data 2. The extensor digitorum longus (EDL) muscles were collected from both ambient temperature housed and 4°C 1 hr exposed ChatCre and cholinergic neuron-specific Tigar knockout (chTKO) mice, and 30 μg of the tissue lysate were used for phospho-Thr172AMPKα (62 kDa) immunoblotting analysis as described in the ‘Immunoblotting’ section.
The raw image was used for Figure 7F. A detailed description of the raw images is shown in Source data 1.