| Cell line (Homo sapiens) |
HaCaT |
Cell Research |
Cat. #: ZQ0044 |
|
| Cell line (Homo sapiens) |
PC-9 |
Cell Bank of Chinese Academy of Sciences |
Cat. #: SCSP-5085CSTR:19375.09.3101HUMSCSP5085 |
|
| Cell line (Homo sapiens) |
THP-1 |
Cell Bank of Chinese Academy of Sciences |
Cat. #: TCHu 57CSTR:19375.09.3101HUMTCHu57 |
|
| Antibody |
Rabbit monoclonal anti-ATGL |
Abcam |
Cat. #: ab109251 |
WB (1:1000) |
| Antibody |
Rabbit monoclonal anti-caveolin-1 |
CST |
Cat. #: 3,267 |
WB (1:1000) |
| Antibody |
Rabbit monoclonal anti-phoso-AKT1 |
CST |
Cat. #: 5,012 |
WB (1:1000) |
| Antibody |
Rabbit monoclonal anti-phoso-AKT2 |
CST |
Cat. #: 8,599 |
WB (1:1000) |
| Antibody |
Rabbit polyclonal anti- phospho-HSL |
Absin |
Cat. #: abs139855 |
WB (1:1000) |
| Antibody |
PE Mouse Monoclonal Anti-rat CD31 |
BD Pharmingen |
Cat. #: 555,027RRID: AB_395657
|
FACS(0.25 µg per million cells in 100 µl volume) |
| Antibody |
FITC Mouse Monoclonal anti-rat CD45 |
BioLegend |
Cat. #: 202,205RRIS: AB_314005 |
FACS(0.25 µg per million cells in 100 µl volume) |
| Antibody |
PerCP/Cyanine5.5 Armenian Hamster Monoclonal Anti-mouse/rat CD29 |
BioLegend |
Cat. #: 102,227RRIS: AB_2572078 |
FACS(0.25 µg per million cells in 100 µl volume) |
| Antibody |
Pe-Cy7 Mouse Monoclonal Anti-mouse/rat/human CD34 |
Santa Cruz Biotechnology |
Cat. #: sc-7324RRIS: AB_2572078 |
FACS(1 µg per million cells in 100 µl volume) |
| Peptide, recombinant protein |
Recombinant human insulin |
Sigma |
Cat. #: 407,709 |
|
| Commercial assay or kit |
NEFA LabAssay |
Wako Diagnostics |
Cat. #: 294–63601 |
|
| Chemical compound, drug |
Liberase TL |
Roche |
Cat. #: 05401020001 |
|
| Chemical compound, drug |
Dexamethasone |
Sigma |
Cat. #: D4902 |
|
| Chemical compound, drug |
Indomethacin |
Sigma |
Cat. #: I8280 |
|
| Chemical compound, drug |
3-isobutyl-1-methylxanthine (IBMX) |
Sigma |
Cat. #: I7018 |
|
| Chemical compound, drug |
BODIPY 493/503 |
ThermoFisher |
Cat. #: D3922 |
|
| Software, algorithm |
Aperio ImageScope |
Leica |
|
|
