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. 2022 Feb 11;11:e70278. doi: 10.7554/eLife.70278

Figure 5. STIM1 colocalizes with BK and TRPM4.

Figure 5.

(A) Total internal reflection fluorescence (TIRF)-mode superresolution localization maps of freshly isolated vascular smooth muscle cells (VSMCs) from control mice immunolabeled for BK (red) and STIM1 (green). Colocalized BK and STIM1 clusters were identified by object-based analysis (OBA) and mapped (cyan). Scale bar: 3 µm. Panels to the right show enlarged areas of the original superresolution maps indicated by the white boxes. Arrows show examples of colocalizing clusters. Scale bar: 500 nm. (B) TIRF-mode superresolution localization maps of freshly isolated VSMCs from control mice immunolabeled for TRPM4 (cyan) and STIM1 (magenta). Colocalized TRPM4 and STIM1 clusters were identified by OBA and mapped (yellow). Scale bar: 2 µm. Panels to the right show enlarged areas of the original superresolution maps indicated by the white boxes. Arrows show examples of colocalizing clusters. Scale bar: 500 nm. (C) Colocalization frequency of BK and STIM1 clusters in imaged cells compared to colocalization frequency of BK and STIM1 clusters in randomized maps generated from respective cells. n = 11 cells from four mice (*p<0.05, paired t-test). (D) Colocalization frequency of TRPM4 and STIM1 clusters in imaged cells compared to colocalization frequency of TRPM4 and STIM1 clusters in randomized maps generated from respective imaged cells (n = 11 cells from four mice; *p<0.05, paired t-test).

Figure 5—source data 1. Individual data points and analysis summaries for datasets shown in Figure 5.