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. 2022 Mar 24;11:e67368. doi: 10.7554/eLife.67368

Figure 6. The molecular signature of sulforaphane (SFN) action in the obese mice.

(A) The expression of NRF2-target gene Hmox1 in the gastrocnemius skeletal muscle (left panel), and eWAT, inguinal white adipose tissue (iWAT), liver, the hypothalamus (right panel) measured by qPCR of diet-induced obese (DIO) wild-type mice (n = 7 per group) following 1 week of vehicle or 5 mg/kg SFN treatments. *p<0.05, calculated by Student’s t-test. (B) Hmox1 mRNA expression measured by qPCR in the skeletal muscle of DIO NRF2 KO mice (n = 8 per group) following i.p. vehicle or 5 mg/kg SFN treatments. (C, D) RNAseq was conducted on DIO wild-type NRF2 KO mouse tissues following vehicle or SFN treatment (n = 5 per tissue, per treatment). (C) Connectivity Map (CMap) correlation score of the SFN gene expression signature from each tissue. (D) KEGG enrichment pathway analysis of skeletal muscle based on the RNAseq results from WT DIO (left panel) and NRF2 KO DIO (right panel) mice.

Figure 6.

Figure 6—figure supplement 1. RNAseq results of the vehicle vs. sulforaphane (SFN)-treated wild-type diet-induced obese (DIO) mice.

Figure 6—figure supplement 1.

(A) Volcano plots for genes at p<0.05 from the RNAseq of liver (Liv, A), the hypothalamus (Hyp, B), skeletal muscle (SkM, C), brown adipose tissue (BAT, D), eWAT (E), and inguinal white adipose tissue (iWAT) (F).
Figure 6—figure supplement 2. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 2.

Dot plots of KEGG Enrichment Pathway analyses of RNA sequencing results indicating significantly up regulated genes following SFN treatments in skeletal muscle of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 3. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 3.

. Dot plots of KEGG, Enrichment Pathway analyses of RNA sequencing results indicating significantly downregulated genes following SFN treatments in skeletal muscle of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 4. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 4.

Dot plots of Reactome Enrichment Pathway analyses of RNA sequencing results indicating significantly up regulated genes following SFN treatments in skeletal muscle of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 5. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 5.

Dot plots of Reactome Enrichment Pathway analyses of RNA sequencing results indicating significantly downregulated genes following SFN treatments in skeletal muscle of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 6. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 6.

Dot plots of GO Enrichment Pathway analyses of RNA sequencing results indicating significantly up regulated genes following SFN treatments in skeletal muscle of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 7. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 7.

Dot plots of GO Enrichment Pathway analyses of RNA sequencing results indicating significantly downregulated genes following SFN treatments in skeletal muscle of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 8. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 8.

Dot plots of KEGG Enrichment Pathway analyses of RNA sequencing results indicating significantly up regulated genes following SFN treatments in liver of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 9. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 9.

Dot plots of KEGG, Enrichment Pathway analyses of RNA sequencing results indicating significantly downregulated genes following SFN treatments in liver of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 10. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 10.

Dot plots of Reactome Enrichment Pathway analyses of RNA sequencing results indicating significantly up regulated genes following SFN treatments in liver of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 11. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 11.

Dot plots of Reactome Enrichment Pathway analyses of RNA sequencing results indicating significantly downregulated genes following SFN treatments in liver of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 12. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 12.

Dot plots of GO Enrichment Pathway analyses of RNA sequencing results indicating significantly up regulated genes following SFN treatments in liver of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 13. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 13.

Dot plots of GO Enrichment Pathway analyses of RNA sequencing results indicating significantly downregulated genes following SFN treatments in liver of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 14. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 14.

Dot plots of KEGG Enrichment Pathway analyses of RNA sequencing results indicating significantly up regulated genes following SFN treatments in inguinal white adipose tissue (iWAT) of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 15. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 15.

Dot plots of KEGG, Enrichment Pathway analyses of RNA sequencing results indicating significantly downregulated genes following SFN treatments in inguinal white adipose tissue (iWAT) of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 16. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 16.

Dot plots of Reactome Enrichment Pathway analyses of RNA sequencing results indicating significantly up regulated genes following SFN treatments in inguinal white adipose tissue (iWAT) of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 17. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 17.

Dot plots of Reactome Enrichment Pathway analyses of RNA sequencing results indicating significantly downregulated genes following SFN treatments in inguinal white adipose tissue (iWAT) of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 18. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 18.

Dot plots of GO Enrichment Pathway analyses of RNA sequencing results indicating significantly up regulated genes following SFN treatments in inguinal white adipose tissue (iWAT) of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 19. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 19.

Dot plots of GO Enrichment Pathway analyses of RNA sequencing results indicating significantly downregulated genes following SFN treatments in inguinal white adipose tissue (iWAT) of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 20. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 20.

Dot plots of KEGG Enrichment Pathway analyses of RNA sequencing results indicating significantly up regulated genes following SFN treatments in epididymal white adipose tissue (eWAT) of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 21. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 21.

Dot plots of KEGG, Enrichment Pathway analyses of RNA sequencing results indicating significantly downregulated genes following SFN treatments in epididymal white adipose tissue (eWAT) of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 22. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 22.

Dot plots of Reactome Enrichment Pathway analyses of RNA sequencing results indicating significantly up regulated genes following SFN treatments in epididymal white adipose tissue (eWAT) of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 23. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 23.

Dot plots of Reactome Enrichment Pathway analyses of RNA sequencing results indicating significantly downregulated genes following SFN treatments in epididymal white adipose tissue (eWAT) of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 24. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 24.

Dot plots of GO Enrichment Pathway analyses of RNA sequencing results indicating significantly up regulated genes following SFN treatments in epididymal white adipose tissue (eWAT) of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 25. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 25.

Dot plots of GO Enrichment Pathway analyses of RNA sequencing results indicating significantly downregulated genes following SFN treatments in epididymal white adipose tissue (eWAT) of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 26. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 26.

Dot plots of KEGG Enrichment Pathway analyses of RNA sequencing results indicating significantly up regulated genes following SFN treatments in hypothalamus of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 27. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 27.

Dot plots of KEGG, Enrichment Pathway analyses of RNA sequencing results indicating significantly downregulated genes following SFN treatments in hypothalamus of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 28. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 28.

Dot plots of Reactome Enrichment Pathway analyses of RNA sequencing results indicating significantly up regulated genes following SFN treatments in hypothalamus of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 29. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 29.

Dot plots of Reactome Enrichment Pathway analyses of RNA sequencing results indicating significantly downregulated genes following SFN treatments in hypothalamus of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 30. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 30.

Dot plots of GO Enrichment Pathway analyses of RNA sequencing results indicating significantly up regulated genes following SFN treatments in hypothalamus of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 31. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO and NRF2 KO DIO mice.

Figure 6—figure supplement 31.

Dot plots of GO Enrichment Pathway analyses of RNA sequencing results indicating significantly downregulated genes following SFN treatments in hypothalamus of WT DIO and NRF2 KO DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 32. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO mice.

Figure 6—figure supplement 32.

Dot plots of KEGG Enrichment Pathway analyses of RNA sequencing results indicating significantly up regulated genes following SFN treatments in brown adipose tissue (BAT) of WT DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 33. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO mice.

Figure 6—figure supplement 33.

Dot plots of KEGG, Enrichment Pathway analyses of RNA sequencing results indicating significantly downregulated genes following SFN treatments in brown adipose tissue (BAT) of WT DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 34. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO mice.

Figure 6—figure supplement 34.

Dot plots of Reactome Enrichment Pathway analyses of RNA sequencing results indicating significantly up regulated genes following SFN treatments in brown adipose tissue (BAT) of WT DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 35. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO mice.

Figure 6—figure supplement 35.

Dot plots of Reactome Enrichment Pathway analyses of RNA sequencing results indicating significantly downregulated genes following SFN treatments in brown adipose tissue (BAT) of WT DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 36. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO mice.

Figure 6—figure supplement 36.

Dot plots of GO Enrichment Pathway analyses of RNA sequencing results indicating significantly up regulated genes following SFN treatments in brown adipose tissue (BAT) of WT DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).
Figure 6—figure supplement 37. Pathway enrichment analyses of bulk RNA sequencing results indicating significantly altered genes following SFN treatments in tissues of WT DIO mice.

Figure 6—figure supplement 37.

Dot plots of GO Enrichment Pathway analyses of RNA sequencing results indicating significantly downregulated genes following SFN treatments in brown adipose tissue (BAT) of WT DIO mice. Pathways enriched in each analysis listed in Y axis. Gene Ratio represents the ratio of differentially expressed genes to all genes for each GO term. Size of dot represents number of significantly altered genes in each condition. Color of dot represents the adjusted p values (purple represents 1 and red represent 0), (Supplementary file 4).