Figure 3.
Tepro fully inhibits IGF-1R-dependent (low-dose) M22 stimulation but only partially inhibits high dose M22 stimulation of hyaluronan secretion that is comprised of IGF1R-dependent and IGF1R-independent stimulating pathways. Dose-response inhibition by Tepro or K1-70 was measured in TEDOFs (see Materials and Methods) stimulated with 0.25 nM (M22low) or 1 nM (M22high) M22. K1-70 was fully efficacious against M22low (black circles) and M22high (black squares). Secreted hyaluronan (HA) was measured in the conditioned media as described in Materials and Methods. Data represent the mean ± SEM of 3 donor TEDOF strains plotted as percent HA levels of the 1 nM M22 response in the absence of antagonistic antibody.