Table 2. True and estimated flux values are compared for a subset of reactions of the E. coli central carbon metabolism network.
The difference is calculated between the true and estimated fluxes. Clearly, eiFlux accurately estimates the fluxes from a simulated set of measurements perturbed by simulated measurement error. Abbreviations: pentose phosphate pathway (PPP), Entner-Doudoroff pathway (EDP), tricarboxylic acid (TCA), glucose 6-phosphate (G6P), fructose 6-phosphate (F6P), glyceraldehyde 3-phosphate (GAP), 1,3-bisphosphoglycerate (BPG), 6-phosphogluconate (6PG), 2-keto-3-deoxy-6-phosphogluconate (KDPG), oxaloacetate (OAA), acetyl-CoA (AcCoA), citrate (Cit), isocitrate (iCit), glyoxylate (Gox), succinate (Suc), malate (Mal), pyruvate (Pyr), phosphoenolpyruvate (PEP), acetate (Ac).
| Pathway | Reaction | True | Estimated | Difference | |
|---|---|---|---|---|---|
| Mean | SD | ||||
| glycolysis | G6P → F6P | 0.7367 | 0.7354 | 0.0295 | 0.0013 |
| glycolysis | GAP → BPG | 1.8510 | 1.8474 | 0.0343 | 0.0037 |
| PPP | 6PG → Ru5P + CO2 | 0.2255 | 0.2219 | 0.0100 | 0.0036 |
| EDP | 6PG → KDPG | 0.0347 | 0.0396 | 0.0370 | 0.0049 |
| TCA cycle | OAA + AcCoA → Cit | 0.0961 | 0.0888 | 0.0112 | 0.0073 |
| glyoxylate shunt | iCit → Gox + Suc | 0.0195 | 0.0166 | 0.0027 | 0.0028 |
| cataplerosis | Mal → Pyr + CO2 | 0.1377 | 0.1111 | 0.0176 | 0.0266 |
| anaplerosis | PEP + CO2 → OAA | 0.1612 | 0.1480 | 0.0196 | 0.0132 |
| efflux | Ac → | 1.6254 | 1.6391 | 0.0088 | 0.0138 |