Figure 2.

Sca1+ mesenchymal stem cells are restricted to the junction of intact skin in injured adult mice and are enriched in the active healing area in young mice. (A) In young animals, the number of Sca1+ mesenchymal cells is significantly less at the junction of intact skin in comparison with the active healing area toward the center of the wound two weeks post-injury (B) In the adult animal, the number of Sca1+ mesenchymal cells is significantly higher at the junction of intact skin (left call out box) in comparison with the active healing area (right call out box) toward the center of the wound (n=5). (C) In vitro scratch assay of BM-MSCs isolated from young vs. adult animals show migration deficiency in the adult group (*P < 0.05, error bars are showing 95% confidence intervals). Arrows indicate Sca1+ cells present in murine dermis.